Physiology of Trees
To study the induction of somatic embryogenesis process in explants of Eucalyptus through chemical / physical / nutritional stimuli.
embryogenesis induction Evaluation of Eucalyptus urophylla.
Assays are performed "in vitro" with different doses and types of phytoregulators and different doses of Ca2 +. There is the absorption of Ca2 + and its relationship with the morphogenetic process. Evaluate the plants are obtained via callus regeneration, as to its multilicação capacity, rooting and growth. The parameters evaluated are: Histological and immunohistochemical: analysis of morphogenetic expression, plant anatomy regenerated via callus, presence of starch, rooting and growth. Biochemical: total proteins (SDS-PAGE), total sugars, peroxidase.Quantitativos: determination of Ca2 + and other ions of interest in the plant material through spectrometry of X-rays.
Determination of enzyme (acid phosphatase and polyamines) on Eucalyptus;
To evaluate the behavior "in vitro" enzyme acid phosphatase activity in Eucalyptus grandis clones by isoenzyme analysis technique, studied the aluminum role in the absorption of the element to the culture medium. Check the variation in the amount existing between Polyamines: Putrescine, spermidine and Spermine due to the effects of aluminum on them.
Different concentrations of aluminum are added to the culture medium for growing "in vitro" Eucalyptus.
Set and setting the pH value of Soluc: will that n & aatilde, the change occurs in aluminum form (affecting abosorção the plant). The parameters evaluated are: Determination of polyamines; Determination of total protein content;
isoenzyme electrophoresis for determination of acid phosphatase;
Biotechnology and micropropagation of ornamental plants;
Studying the process of micropropagation of conifers (Cupressus macrocarpa and Pinus silvestris) and set an annual fertilization program for Pinus silvestris and Cyptomeria japonica.
Promote micropropagation cupresus macrocarpa adjusting the culture medium.
Calculation and determination of an optimal program of fertilization through theories formulated to obtain conifers used as Christmas trees and micropropagation through explants of young plants.
Mineral Nutrition, Physiology and Plant Propagation
Develop basic and applied research related to the cultivation of tropical species "in vitro", aiming to improve management techniques and cultivation of these species.
Use of stable isotopes (15N) to study the absorption of nitrogen by bromeliads cultured "in vitro".
Using cultured Eucalyptus nutrient content analysis "ex vitro", for the formulation of culture media for in vitro culture.
Effect of calcium levels in morphogenesis of Eucalyptus sp yolks cultured "in vitro".
Effect of magnesium levels in the morphogenesis of Cryptanthus sp gems (bromeliaceae) cultured "in vitro".
Effect of relationships NH4 / NO3, P / N and total N content in absorption of nutrients in Aechemea nudicaulis (bromeliaceae) cultivated "in vitro".
Effect of NH4 / NO3 relations, nitrate reductase activity and the phenolic content and free ammonia in Aechemea nudiculis (bromeliaceae) cultivated "in vitro".
basic fertilization effect on growth, mineral composition and biochemical parameters Aechemea bromelifoia (bromeliaceae) grown under four different formulations of substrate, in "floating" system.
Analysis of growth of three species of Cryptanthus (bromeliaceae) in different organic substrates.
Stress in Plants morphophysiology
Develop studies aimed at evaluating abiotic stresses induced in plants "in vitro", aimed at establishing morphophysiological parameters for pre-selection and breeding of matrices.
Assays are performed "in vitro" with changes in osmotic potential of the PEG culture media for inducing water stress. Induction temperature variations, nutritional, pH and salinity in culture conditions "in vitro" and in the field. The parameters evaluated are: Biochemical: total proteins on SDS-PAGE, proline content of nonstructural total sugar content of chlorophyll A and B. Immuno assays Western Blott technique (in development). Morphophysiological: dry matter yield, plant height, leaf anatomy and external morphology.